Journal: CytoJournal

Article Title: Mechanism of 15-hydroxyprostaglandin dehydrogenase protein inhibiting cervical cancer cell proliferation through downregulation of the notch1 signaling pathway

doi: 10.25259/cytojournal_57_2025

Figure Lengend Snippet: Figure 1: 15-PGDH downregulation and activation of the Notch1 signaling pathway in human CC cells. (a) WB analysis of the expression levels of 15-PGDH and Notch1 signaling pathway- related proteins in HcerEpiC and CC cells (HeLa, Caski, ME180). (b-e) Quantitative analysis of the protein levels of 15-PGDH and Notch1 signaling pathway-related proteins. (f) RT-qPCR analysis of 15-PGDH messenger RNA levels in the cells. n = 3, *P < 0.05, **P < 0.01, ***P < 0.001 versus HcerEpiC. HcerEpiC: Human cervical epithelial cells, HeLa: Henrietta Lacks cells, Caski: Cervical squamous carcinoma cells, ME180: Human cervical epidermoid carcinoma cells, 15-PGDH: 15-hydroxyprostaglandin dehydrogenase, Notch1: Notch receptor 1, Jagged1: Jagged canonical Notch ligand 1, Hes1: Hes family bHLH transcription factor 1, GAPDH: Glyceraldehyde-3-phosphate dehydrogenase, CC: Cervical cancer, WB: Western blotting.

Article Snippet: Cell counting kit-8 (CCK-8) assay The survival rate of the HeLa and Caski cells was evaluated utilizing the CCK-8 kit (C0038, Beyotime, Shanghai, China).

Techniques: Activation Assay, Expressing, Quantitative RT-PCR, Western Blot

Journal: CytoJournal

Article Title: Mechanism of 15-hydroxyprostaglandin dehydrogenase protein inhibiting cervical cancer cell proliferation through downregulation of the notch1 signaling pathway

doi: 10.25259/cytojournal_57_2025

Figure Lengend Snippet: Figure 2: Effect of 15-PGDH protein or its inhibitor on Notch1 signaling pathway activation in HeLa and Caski Cells. (a) WB assessment of 15-PGDH and Notch1 pathway proteins expression in HeLa cells. (b-e) Quantitative assessment of 15-PGDH and Notch1 pathway proteins amounts in HeLa cells. (f) WB assessment of 15-PGDH and Notch1 pathway proteins expression in Caski cells. (g-j) Quantitative assessment of 15-PGDH and Notch1 pathway proteins amounts in Caski cells. n = 3, *P < 0.05, **P < 0.01, ***P < 0.001 versus control. HeLa: Henrietta Lacks cells, Caski: Cervical squamous carcinoma cells, 15-PGDH: 15-hydroxyprostaglandin dehydrogenase, Notch1: Notch receptor 1, Jagged1: Jagged canonical Notch ligand 1, Hes1: Hes family bHLH transcription factor 1, GAPDH: Glyceraldehyde-3-phosphate dehydrogenase, WB: Western blotting.

Article Snippet: Cell counting kit-8 (CCK-8) assay The survival rate of the HeLa and Caski cells was evaluated utilizing the CCK-8 kit (C0038, Beyotime, Shanghai, China).

Techniques: Activation Assay, Expressing, Control, Western Blot

Journal: CytoJournal

Article Title: Mechanism of 15-hydroxyprostaglandin dehydrogenase protein inhibiting cervical cancer cell proliferation through downregulation of the notch1 signaling pathway

doi: 10.25259/cytojournal_57_2025

Figure Lengend Snippet: Figure 3: 15-PGDH protein inhibits Notch1 overexpression-induced proliferation of HeLa and Caski cells. (a) WB analysis of Notch1 overexpression in HeLa and Caski cells. (b) Quantitative assessment of Notch1 overexpression abundance in HeLa and Caski cells. (c and d) CCK-8 assay to detect the proliferation of HeLa and Caski cells. (e) Transwell migration assay to assess the migration ability of HeLa and Caski cells. Magnification: ×200, scale bar: 50 μm. (f and g) Statistical outcomes of the Transwell migration test. (h) EdU assay analysis of the HeLa cell proliferation rate. Magnification: ×200, scale bar: 50 μm. (i) Statistical analysis of the EdU-positive rate in HeLa cells. (j) EdU assay analysis of the Caski cell proliferation rate. Magnification: ×200, scale bar: 50 μm. (k) Statistical analysis of the EdU-positive rate in Caski cells. (l and m) Caspase activity in HeLa and Caski cells was detected using a caspase assay kit. n = 3, **P < 0.01 and ***P < 0.001 versus Control; ###P < 0.001 versus 15-PGDH; ^^P < 0.01 and ^^^P < 0.001 versus Notch1-OE. HeLa: Henrietta Lacks cells, Caski: cervical squamous carcinoma cells, 15-PGDH: 15-hydroxyprostaglandin dehydrogenase, Notch1: Notch receptor 1, GAPDH: Glyceraldehyde-3- phosphate dehydrogenase, EdU: 5-ethynyl-2’-deoxyuridine, DAPI: 4′,6-diamidino-2-phenylindole, Caspase-3: Cysteine-aspartic protease 3, Caspase-8: Cysteine-aspartic protease 8, and Caspase-9: Cysteine-aspartic protease 9, WB: Western blotting, CCK-8: Cell counting kit-8.

Article Snippet: Cell counting kit-8 (CCK-8) assay The survival rate of the HeLa and Caski cells was evaluated utilizing the CCK-8 kit (C0038, Beyotime, Shanghai, China).

Techniques: Over Expression, CCK-8 Assay, Transwell Migration Assay, Migration, EdU Assay, Activity Assay, Caspase Assay, Control, Western Blot, Cell Counting

Journal: CytoJournal

Article Title: Mechanism of 15-hydroxyprostaglandin dehydrogenase protein inhibiting cervical cancer cell proliferation through downregulation of the notch1 signaling pathway

doi: 10.25259/cytojournal_57_2025

Figure Lengend Snippet: Figure 4: 15-PGDH protein inhibits Notch activation induced by Notch1 overexpression in HeLa and Caski cells. (a) WB analysis of 15-PGDH and proteins associated with the Notch1 pathway in HeLa cells. (b-e) Quantitative assessment of 15-PGDH and Notch1 pathway-related proteins expression levels. (f) WB analysis of 15-PGDH and proteins associated with the Notch1 pathway in Caski cells. (g-j) Quantitative assessment of 15-PGDH and Notch1 pathway-related protein expression levels. n = 3, ***P < 0.001 versus Control;

Article Snippet: Cell counting kit-8 (CCK-8) assay The survival rate of the HeLa and Caski cells was evaluated utilizing the CCK-8 kit (C0038, Beyotime, Shanghai, China).

Techniques: Activation Assay, Over Expression, Expressing, Control